Multi-omics of Two-Day CPET Plasma and Immune Cells

In order to investigate the phenomenon of post-exertional malaise in ME/CFS, the Cornell NIH ME/CFS Center is collecting blood before and after subjects perform two successive maximal cardiopulmonary exercise tests (CPETs) separated by 24 hrs. The maximal CPETs are not the informative invasive CPETs used by the Harvard Center. Nevertheless, these standard CPETs provide information about VO2max, ventilatory threshold, abnormalities in blood pressure and heart rate response, and the effect of the effort of the first CPET on performance of a second CPET 24 hrs later. The CPETs are being performed in three locations: Weill Cornell Medicine, Ithaca College, and by Workwell Foundation at Dr. John Chia’s clinic in Torrance, CA. Sedentary behavior is required for inclusion of healthy control subjects. The blood is processed into plasma, serum, and PBMCs the same day as testing, stored at -80o C and then shipped to Cornell for storage at -80o C. or in liquid nitrogen. Proteins, metabolites, antibodies, and extracellular vesicles are being measured in plasma/serum by a variety of methods. Immune cells are undergoing analysis by single-cell RNA sequencing, single-cell ATACseq, bisulphite sequencing, CYTOF and flow cytometry.